The kit provides an optimized reagent and buffer system for efficient extraction of biologically active and soluble total protein from yeast cells. The kit contains EDTA 、CaCl2 , DTT, protease inhibitors, as well as a proprietary reagent to break cell wall. Native and/or recombinant proteins extracted are preserved with biological activity integrities and are suitable for many downstream applications including SDS-PAGE, Western blot, enzyme analysis, 2D gel electrophoresis. The kit is sufficient for 50x50 mg wet yeast cells
產品名稱 : Yeast Protein Extraction kit-20R
產品編碼 : Bio-PYF-1k
產品規格 : 1Kit
Components
Isoosmotic Buffer 75ml
Snailase Buffer 250ul
Hypoosmotic Buffer 25ml
PMSF Solution 250ul
Storage
Transportation at room temperature. Upon receipt, store Isoosmotic Buffer, Hypoosmotic Buffer at 4oC. Store the Snailase buffer and PMSF Buffer at -20oC.
