From outside to inside, living cells can divided into three major parts: membrane, cytosol, and nucleus part. The traffic of proteins between these parts are well known mechanism of transduce and maintaining intracellular signaling, especially when response to environment stimuli. Therefore, investigators are getting more interests on protein localization between these parts. Nuclear fractionation kit is a dramatic tool that provide a conveniently, efficiently step to subfractionate membrane, cytosol, and nucleus part to performing related biomedical analysis in a short time without use any density ultracentrifugation. It is designed base on the differential lysis technology, that lead to differentially extract the membrane, cytosol, and nucleus part of the cells by low speed centrifugation. Please following the protocol then you may be feeling easy to get subfraction extracts in your studies.
產品名稱 : Cell Nuclear Protein Extraction kit-20R
產品編碼 : Bio-PNF-20
產品規格 : 20R
Nuclear fractionation kit is designed for extraction of membrane protein fractions from adherent and suspension cultured mammalian cells. All process takes less than 1 hour and it’s very simple to use. This kit provide high quality of protein fractionation and fit for many downstream applications.
Suitable for many downstream applications such as 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.